But as O and X are routinely used on this site to indicate passable and impassable tiles, this is confusing.
So are you asking if you can have a sprite that is 96 pixels wide and 64 pixels high? If that is what you want, the answer is yes if, and only if, you format a sprite sheet correctly, so that the 12 images (3 down, 3 left, 3 right, 3 up) are arranged in 3 columns and 4 rows. They have to be evenly distributed, so your sprite sheet will measure in total 288 pixels wide and 256 pixels high. The file name must begin with the symbol $ so that the engine knows this is a single sprite sheet, and not a normal 8 character sprite sheet.
If that isn't what you were asking, then you will have to explain more clearly what you want.